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Image Search Results
Journal: Frontiers in Molecular Neuroscience
Article Title: The Synaptic Dysregulation in Adolescent Rats Exposed to Maternal Immune Activation
doi: 10.3389/fnmol.2020.555290
Figure Lengend Snippet: Maternal immune activation stimulates an increase of inflammatory markers in hippocampi of adolescent male rats. LPS (100 μg/kg b.w.) was injected intraperitoneally at gestation day 9.5 to female rats. Offspring male rats at day 52 were decapitated, and the hippocampi were collected. (A) Densitometric analysis of immunoreactivity of Iba1 ( n = 3 and 4), s100β ( n = 5 and 4), arginase ( n = 5 and 4), and iNOS ( n = 3 and 4). Data were normalised to GAPDH immunoreactivity. (B) Representative immunoblots of Iba1, s100β arginase and iNOS, including GAPDH as a loading control. Each sample presented on representative pictures is from separate animal. (C) The level of IFN-γ in hippocampal extract, as determined using the Bio-Plex Pro Rat Cytokine 23-Plex Assay on Luminex Bio-Plex 200 system ( n = 7). * p < 0.05, ** p < 0.01, compared with the control group.
Article Snippet: Chemiluminescent reagent Clarity Western ECL Substrate and
Techniques: Activation Assay, Injection, Western Blot, Plex Assay, Luminex